Staff Associates/Research Interns, & Graduate Students

Tracy Preko, B.S.

I joined the John Lab as a research aide after I graduated from the University of Notre Dame in May of 2021 with a B.S. in Neuroscience and Behavior along with a minor in Poverty Studies. During my time here, I am excited to gain in-depth knowledge into the development of glaucoma and how it is investigated in mice models while gaining experience in various techniques and procedures like genotyping, animal husbandry, immunohistochemistry, imaging, ocular examinations and physiology. My hobbies include exploring new recipes and dishes while catching up on my favorite shows and movies. I am learning a lot and gaining skills and was promoted to Staff Associate in the summer of 2022.

Research Associate II

Felicia Juarez, B.S.

I received my Bachelor of Science in Neuroscience with a concentration in Molecular and Cellular Biology from the Johns Hopkins University in 2017. My main research interests are genetics and neuroscience. As an undergraduate, I worked in Seth Blackshaw's lab on a project entitled "Investigating the role of Lhx2 in the development of the ciliary body." Since graduating college, I have worked in several labs ranging from studying blood diseases to building a library of nuclear constructs to study the phase separation of nuclear proteins in vivo. I am experienced in tissue culture, mouse husbandry, cloning, and various molecular biology techniques. I joined the John lab in June of 2020 as a Research Associate. During my free time I enjoy reading, exploring new areas in New York, and spending time with my dog, Nova.

Nicholas Tolman B.A., Ph.D student

I received my B.A. in Behavioral Neuroscience from Connecticut College . During my time at Connecticut College, I worked on several different research projects. We studied whether Ceftriaxone and other β-lactam antibiotic compounds could attenuate morphine reward in rats by enhancing the reuptake rate of excitatory amino-acid transporters. For my honors thesis, I examined the effects of developmental lead exposure on working memory performance in rats after manipulating several elements of the animal’s developmental environment.

I started my Ph.D. in the John lab in 2016. I am studying the roles of a LIM domain transcription factor , LMX1b, in ocular development and adult-onset glaucoma. This has allowed me an unusually broad graduate training including clinical, molecular, genetic, genomic and other computational/statistical approaches using R, Seurat and other software . Beyond immunohistochemistry and other standard molecular techniques, I have mastered various delicate dissections (retina, optic nerve, brain regions and ocular drainage tissues) and precise ocular physiological measurements (including intraocular pressure and ocular fluid drainage or outflow). My projects have also involved clinical and histologic examination of mouse eyes and Mendelian and complex genetics including the diversity outbred mouse population. I have been involved with several other exciting projects, including the screening for and characterization of high IOP mutants and in a collaboration with engineers towards developing new treatment tools. Currently, I am focusing on the developmental biology of ocular development in Lmx1b mutants as well as the gene expression networks controlling ocular development and IOP elevation (using single cell sequencing, bulk RNA sequencing and other approaches). Dr. John has facilitated my interaction with collaborators and exposed me to grant writing, lab. management decisions and strategic planning. This diverse training along with his mentorship and the lab's environment are putting me in a strong position for my future career as a PI.

Being from coastal Maine, I was used to being an outdoor person at our lab's initial location next to Acadia National Park. The exciting and fast paced environment at our new location, Columbia University, is an amazing experience with the opportunity to learn from many first rate scientists.